Micron-scale Intrashell Carbon Isotope and trace element data in the Cultured Planktic Foraminifer O. universa Vetter, L.; Kozdon, R.; Valley, J.W.; Mora, C.I.; Spero, H.J. Vetter, L. Kozdon, R. Valley, J.W. Mora, C.I. Spero, H.J. earth science/paleoclimate/paleoceanography (notes,null,null,null,null,paleoceanography,null,null,C,individual foraminifera specimen) earth science/paleoclimate/paleoceanography (delta 13C,Orbulina universa,two standard deviations lower bound,per mil VPDB,null,paleoceanography,null,secondary ion mass spectrometry,N,calcite test (shell)) earth science/paleoclimate/paleoceanography (notes,null,null,null,null,paleoceanography,null,null,C,individual spot measurements of delta 18O in specimens) earth science/paleoclimate/paleoceanography (notes,null,null,null,null,paleoceanography,null,null,C,Identifies whether calcite was precipitated in ambient (A) or labeled (L) seawater) earth science/paleoclimate/paleoceanography (notes,null,null,null,null,paleoceanography,null,null,N,Distance of the center of a 3 µm spot from the inner shell surface of an individual foraminifer specimen) earth science/paleoclimate/paleoceanography (delta 13C,Orbulina universa,null,per mil VPDB,null,paleoceanography,null,secondary ion mass spectrometry,N,in situ measurements of 3 µm domains within calcite shell) earth science/paleoclimate/paleoceanography (delta 13C,Orbulina universa,two standard deviations upper bound,per mil VPDB,null,paleoceanography,null,secondary ion mass spectrometry,N,calcite test (shell)) earth science/paleoclimate/paleocean earth science/paleoclimate/paleocean earth science/paleoclimate/paleocean (Santa Catalina Island>LATITUDE 33.26>LONGITUDE 118.29) In this study, we present experimental results from the planktic foraminifer Orbulina universa, cultured in the laboratory. We demonstrate that the d13C of shell calcite precipitated in 13C-labeled seawater for 24 h can be resolved and accurately measured using Secondary Ion Mass Spectrometry (SIMS). Specimens maintained at 20 °C were transferred from ambient seawater (d13CDIC = +1.3‰) into seawater with d13CDIC = +51.5‰ and enriched [Ba2+] for 24 h. Specimens were then transferred into ambient seawater with elevated [87Sr] for 6–9 h of calcification, followed by a transfer back into unlabeled ambient seawater until gametogenesis. This technique produced O. universa shells with calcite layers of distinct geochemical signatures. We quantify the spatial positions of trace element labels in the shells using laser ablation ICP-MS depth profiling. Using fragments from the same shells, we quantify intrashell d13Ccalcite using SIMS with a 6 or 8 µm spot (2 SD range ±0.5‰ to 1.7‰). Measured d13Ccalcite values in O. universa shell layers precipitated in ambient seawater are within 2‰ of predicted d13Ccalcite values. In 13C-labeled bands of calcite, 6 µm SIMS spot measurements are within 2‰ of predicted d13Ccalcite values, whereas 8 µm SIMS spots yield intermediate, mixed values. The spatial agreement between trace element and carbon isotope data suggests that 13C and cation tracers are synchronously incorporated into shell calcite. These results demonstrate the ability of SIMS d13C measurements to resolve ~10 µm features in foraminifer shell calcite using a 6 µm spot, and highlight the potential of this technique for addressing questions about ecology, biomineralization, and paleoceanography. NCDC-Paleoclimatology https://www.ncdc.noaa.gov/data-access/paleoclimatology-data - Bauer Bruce Investigator : Vetter, L. Investigator : Kozdon, R. Investigator : Valley, J.W. Investigator : Mora, C.I. Investigator : Spero, H.J. 2016-09-30 ONLINE Files online, ASCII https://www.ncdc.noaa.gov/paleo/study/20549 https://www1.ncdc.noaa.gov/pub/data/paleo/contributions_by_author/vetter2014/vetter2014.txt https://www1.ncdc.noaa.gov/pub/data/paleo/contributions_by_author/vetter2014/vetter2014.txt English https://www.ncdc.noaa.gov/paleo/study/20549 https://www1.ncdc.noaa.gov/pub/data/paleo/contributions_by_author/vetter2014/vetter2014.txt Vetter, L., R. Kozdon, J.W. Valley, C.I. Mora, and H.J. Spero2014SIMS measurements of intrashell d13C in the cultured planktic foraminifer Orbulina universaGeochimica et Cosmochimica Acta139527-53910.1016/j.gca.2014.04.049 118.29 33.26 118.29 33.26