noaa-coral-22230 Monterey Bay Bamboo Coral phenylalanine d15N Data from 1977-2004 CE Broek, T.A.B.; Walker, B.D.; Andreasen, D.H.; McCarthy, M.D. Monterey Bay Bamboo Coral phenylalanine d15N Data from 1977-2004 CE 2017-06-06 NCDC-Paleoclimatology ONLINE Files Pending https://www.ncdc.noaa.gov/paleo/study/22230 Investigator T.A.B. Broek Investigator B.D. Walker Investigator D.H. Andreasen Investigator M.D. McCarthy earth science paleoclimate corals and sclerosponges depth at sample start,null,null,millimeter,null,corals and sclerosponges,null,null,N,null earth science paleoclimate corals and sclerosponges age,null,null,year Common Era,null,corals and sclerosponges,null,null,N,14C age model mean earth science paleoclimate corals and sclerosponges depth,null,null,millimeter,null,corals and sclerosponges,null,null,N,mean distance from outer margin of section earth science paleoclimate corals and sclerosponges delta 15N,Isidella sp.,null,per mil AIR,null,corals and sclerosponges,raw,elemental analysis|isotope ratio mass spectrometry,N,null earth science paleoclimate corals and sclerosponges delta 15N,Isidella sp.|phenylalanine,null,per mil AIR,null,corals and sclerosponges,raw,gas chromatography|isotope ratio mass spectrometry,N,Used GC-Combustion-IRMS earth science paleoclimate corals and sclerosponges sample identification,null,null,null,null,corals and sclerosponges,null,null,N,null earth science paleoclimate corals and sclerosponges delta 15N,Isidella sp.|phenylalanine,null,per mil AIR,null,corals and sclerosponges,raw,elemental analysis|isotope ratio mass spectrometry,N,first purified via high-pressure liquid chromatography earth science paleoclimate corals and sclerosponges depth at sample end,null,null,millimeter,null,corals and sclerosponges,null,null,N,null earth science paleoclimate corals and sclerosponges chemistry geoscientificInformation 1977 AD 2004 AD -27 cal yr BP -54 cal yr BP Complete 36.74423 36.74423 -122.037215 -122.037215 -870 -870 Continent North America United States Of America California Monterey Bay T1104 A7>LATITUDE 36.74423>LONGITUDE -122.037215 None Please cite original publication, online resource, dataset and publication DOIs (where available), and date accessed when using downloaded data. If there is no publication information, please cite investigator, title, online resource, and date accessed. The appearance of external links associated with a dataset does not constitute endorsement by the Department of Commerce/National Oceanic and Atmospheric Administration of external Web sites or the information, products or services contained therein. For other than authorized activities, the Department of Commerce/NOAA does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this Department of Commerce/NOAA Web site. English DOC/NOAA/NESDIS/NCEI National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce https://www.ncdc.noaa.gov/data-access/paleoclimatology-data DATA Center Contact Bruce Bauer bruce.a.bauer@noaa.gov paleo@noaa.gov 303-497-6280 303-497-6513

325 Broadway, E/NE31

Boulder CO 80305-3328 USA online ASCII Broek, T.A.B., B.D. Walker, D.H. Andreasen, and M.D. McCarthy 2013 Rapid Communications in Mass Spectrometry 27 2327-2337 10.1002/rcm.6695 http://onlinelibrary.wiley.com/doi/10.1002/rcm.6695/full

RATIONALE: Compound-specific isotope analysis of individual amino acids (CSI-AA) is a powerful new tool fortracing nitrogen (N) source and transformation in biogeochemical cycles. Specifically, the d15N value of phenylalanine (d15NPhe) represents an increasingly used proxy for source d15N signatures, with particular promise for paleoceanographic applications. However, current derivatization/gas chromatography methods require expensive and relatively uncommon instrumentation, and have relatively low precision, making many potential applications impractical. METHODS: A new offline approach has been developed for high-precision d15N measurements of amino acids (d15NAA), optimized for d15NPhe values. Amino acids (AAs) are first purified via high-pressure liquid chromatography (HPLC), using a mixed-phase column and automated fraction collection. The d15N values are determined via offline elemental analyzer-isotope ratio mass spectrometry (EA-IRMS). RESULTS: The combinedHPLC/EA-IRMSmethod separated most proteinAAs with sufficient resolution to obtain accurate d15N values, despite significant intra-peak isotopic fractionation. For d15NPhe values, the precision was ±0.16‰ for standards, 4Å~ better than gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS; ±0.64‰). We also compared a d15NPhe paleo-record from a deep-sea bamboo coral from Monterey Bay, CA, USA, using our method versus GC/C/IRMS. The two methods produced equivalent d15NPhe values within error; however, the d15NPhe values from HPLC/EA-IRMS had approximately twice the precision of GC/C/IRMS (average stdev of 0.27‰±0.14‰ vs 0.60‰±0.20‰, respectively). CONCLUSIONS: These results demonstrate that offline HPLC represents a viable alternative to traditional GC/C/IMRS for d15NAA measurement. HPLC/EA-IRMS is more precise and widely available, and therefore useful in applications requiring increased precision for data interpretation (e.g. d15N paleoproxies). STUDY NOTES: Measurement precision- bulk d15N: <0.1 per mil AIR; d15N_Phe (GC-C-IRMS): 0.6 per mil AIR; d15N_Phe (HPLC-EA-IRMS): < 0.3 per mil AIR

GET DATA https://www1.ncdc.noaa.gov/pub/data/paleo/coral/east_pacific/broek2013/broek2013.txt NOAA Template File; d15N Data USA/NOAA DIF Version 9.8.4 2018-12-11 2018-12-11