noaa-ocean-22233 Santa Barbara Basin Bulk and Compound Specific Nitrogen Isotope Data Batista, F.C.; Ravelo, A.C.; Crusius, J.; Casso, M.A.; McCarthy, M.D. Santa Barbara Basin Bulk and Compound Specific Nitrogen Isotope Data 2017-06-12 NCDC-Paleoclimatology ONLINE Files Pending https://www.ncdc.noaa.gov/paleo/study/22233 Investigator F.C. Batista Investigator A.C. Ravelo Investigator J. Crusius Investigator M.A. Casso Investigator M.D. McCarthy earth science paleoclimate paleoceanography delta 15N,glutamic acid|glutamine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,lysine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,phenylalanine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,serine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,threonine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,tyrosine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography carbon,null,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,lipid fraction earth science paleoclimate paleoceanography carbon,amino acid,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,total hydrolysable amino acid fraction earth science paleoclimate paleoceanography nitrogen,sediment,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,bulk material earth science paleoclimate paleoceanography nitrogen,null,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,acid insoluble fraction earth science paleoclimate paleoceanography glycine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography leucine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography serine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography delta 13C,sediment,null,per mil VPDB,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,bulk material earth science paleoclimate paleoceanography delta 15N,amino acid,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,total hydrolysable amino acid; See original publication for parameter formulation earth science paleoclimate paleoceanography delta 15N,null,null,per mil AIR,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,bound fraction earth science paleoclimate paleoceanography alanine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography glutamic acid and glutamine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,leucine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 13C,null,null,per mil VPDB,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,bound fraction earth science paleoclimate paleoceanography carbon,sediment,null,weight percent,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,bulk material earth science paleoclimate paleoceanography carbon,null,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,bound fraction earth science paleoclimate paleoceanography collection water depth,null,null,meter,null,paleoceanography,null,null,N,null earth science paleoclimate paleoceanography lysine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography delta 13C,organic matter,null,per mil VPDB,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,organic carbon earth science paleoclimate paleoceanography delta 15N,sediment,null,per mil AIR,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,bulk fraction earth science paleoclimate paleoceanography organic carbon,null,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography nitrogen,amino acid,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,total hydrolysable amino acid fraction earth science paleoclimate paleoceanography nitrogen,null,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,bound fraction earth science paleoclimate paleoceanography isoleucine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography phenylalanine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,alanine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,valine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 13C,null,null,per mil VPDB,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,acid soluble fraction earth science paleoclimate paleoceanography delta 13C,null,null,per mil VPDB,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,acid insoluble fraction earth science paleoclimate paleoceanography depth,null,null,centimeter,null,paleoceanography,null,null,N,Multicore Depth earth science paleoclimate paleoceanography valine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,glycine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,sediment,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 13C,null,null,per mil VPDB,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,lipid fraction earth science paleoclimate paleoceanography delta 15N,amino acid,null,per mil AIR,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,total hydrolysable amino acid fraction earth science paleoclimate paleoceanography carbon,null,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,acid soluble fraction earth science paleoclimate paleoceanography carbon,null,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,acid insoluble fraction earth science paleoclimate paleoceanography proline,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography threonine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography tyrosine,null,null,mole percent,null,paleoceanography,null,gas chromatography|mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,asparagine|aspartic acid,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,isoleucine,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,proline,null,per mil AIR,null,paleoceanography,null,gas chromatography|isotope ratio mass spectrometry,N,null earth science paleoclimate paleoceanography delta 15N,null,null,per mil AIR,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,acid soluble fraction earth science paleoclimate paleoceanography delta 15N,null,null,per mil AIR,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,acid insoluble fraction earth science paleoclimate paleoceanography nitrogen,null,null,weight percent,null,paleoceanography,null,elemental analysis|isotope ratio mass spectrometry,N,acid soluble fraction earth science paleoclimate paleocean nitrogen isotopes geoscientificInformation Complete 34.233333 34.233333 -120.016667 -120.016667 Ocean Pacific Ocean Eastern Pacific Ocean Santa Barbara Basin>LATITUDE 34.233333>LONGITUDE -120.016667 None Please cite original publication, online resource, dataset and publication DOIs (where available), and date accessed when using downloaded data. If there is no publication information, please cite investigator, title, online resource, and date accessed. The appearance of external links associated with a dataset does not constitute endorsement by the Department of Commerce/National Oceanic and Atmospheric Administration of external Web sites or the information, products or services contained therein. For other than authorized activities, the Department of Commerce/NOAA does not exercise any editorial control over the information you may find at these locations. These links are provided consistent with the stated purpose of this Department of Commerce/NOAA Web site. English DOC/NOAA/NESDIS/NCEI National Centers for Environmental Information, NESDIS, NOAA, U.S. Department of Commerce https://www.ncdc.noaa.gov/data-access/paleoclimatology-data DATA Center Contact Bruce Bauer bruce.a.bauer@noaa.gov paleo@noaa.gov 303-497-6280 303-497-6513

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Boulder CO 80305-3328 USA online ASCII Batista, Fabian C., A. Christina Ravelo, John Crusius, Michael A. Casso, and Matthew D. McCarthy 2014 Geochimica et Cosmochimica Acta 142 553-569 10.1016/j.gca.2014.08.002 http://www.sciencedirect.com/science/article/pii/S0016703714004979

The nitrogen (N) isotopic composition (d15N) of bulk sedimentary N (d15Nbulk) is a common tool for studying past biogeochemical cycling in the paleoceanographic record. Empirical evidence suggests that natural fluctuations in the d15N of surface nutrient N are reflected in the d15N of exported planktonic biomass and in sedimentary d15Nbulk. However, d15Nbulk is an analysis of total combustible sedimentary N, and therefore also includes mixtures of N sources and/or selective removal or preservation of N-containing compounds. Compound-specific nitrogen isotope analyses of individual amino acids (d15NAA) are novel measurements with the potential to decouple d15N changes in nutrient N from trophic effects, two main processes that can influence d15Nbulk records. As a proof of concept study to examine how d15NAA can be applied in marine sedimentary systems, we compare the d15NAA signatures of surface and sinking POM sources with shallow surface sediments from the Santa Barbara Basin, a sub-oxic depositional environmental that exhibits excellent preservation of sedimentary organic matter. Our results demonstrate that d15NAA signatures of both planktonic biomass and sinking POM are well preserved in such surface sediments. However, we also observed an unexpected inverse correlation between d15N value of phenylalanine (d15NPhe; the best AA proxy for N isotopic value at the base of the food web) and calculated trophic position. We used a simple N isotope mass balance model to confirm that over long time scales, d15NPhe values should in fact be directly dependent on shifts in ecosystem trophic position. While this result may appear incongruent with current applications of d15NAA in food webs, it is consistent with expectations that paleoarchives will integrate N dynamics over much longer timescales. We therefore propose that for paleoceanographic applications, key d15NAA parameters are ecosystem trophic position, which determines relative partitioning of 15N into source AA versus trophic AA pools, and the integrated d15NAA of all common protein AA (d15NTHAA), which serves as a proxy for the d15N of nutrient N. Together, we suggest that these can provide a coupled picture of regime shifts in planktonic ecosystem structure, d15N at the base of food webs, and possibly additional information about nutrient dynamics. STUDY NOTES: Bulk and compound specific nitrogen isotopes in shallow surface sediments from Santa Barbara Basin Provided Keywords: Santa Barbara Basin, Nitrogen Isotopes, amino acids

GET DATA https://www1.ncdc.noaa.gov/pub/data/paleo/contributions_by_author/batista2014/batista2014-aa.txt NOAA Template File; Amino Acid mol% Data GET DATA https://www1.ncdc.noaa.gov/pub/data/paleo/contributions_by_author/batista2014/batista2014-wtpct.txt NOAA Template File; weight percent N and C by fraction GET DATA https://www1.ncdc.noaa.gov/pub/data/paleo/contributions_by_author/batista2014/batista2014-isofract.txt NOAA Template File; d15N and d13C by fraction GET DATA https://www1.ncdc.noaa.gov/pub/data/paleo/contributions_by_author/batista2014/batista2014-d15n-aa.txt NOAA Template File; d15N Amino Acid and Bulk USA/NOAA DIF Version 9.8.4 2018-12-11 2018-12-11